Re-infection profile of baculoviruses to Sf-9 insect cells that have already been infected: Virus binding and recombinant protein production

Takeshi Gotoh, Nozomi Ando, Ken Ichi Kikuchi

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Baculovirus re-infection to already-infected insect cells was assessed by comparing the binding of radio-labeled AcMNPV to Sf-9 cells with the expression of marker proteins, β-galactosidase and variant of green fluorescent protein, respectively coded in the baculoviruses. The binding of baculoviruses to virus-infected Sf-9 insect cells was significantly inhibited with the elapse of time post primary infection (ppi) and completely inhibited by 12 h-ppi. The protein analysis suggested that baculoviruses bound to already-infected cells were internalized into the cells and routed for protein expression. However, the yield of protein coded in 2nd viruses (for re-infection) decreased with delaying re-infection time from the infection with primary viruses, and the expression of 2nd virus protein was not determined on the reinfection at 12 h-ppi. On the contrary, the expression of primary virus protein was inhibited by bringing forward the time of re-infection with 2nd viruses, giving nearly a steady level of total volumetric productivity.

Original languageEnglish (US)
Pages (from-to)804-808
Number of pages5
JournalJournal of Chemical Engineering of Japan
Volume41
Issue number8
DOIs
StatePublished - Sep 24 2008

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Keywords

  • AcMNPV
  • Baculovirus
  • Radiolabeling
  • Re-infection
  • Sf-9

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