Rapid loss of translatable messenger RNA of phosphoenolpyruvate carboxykinase during glucose repression in liver

S. M. Tilghman, R. W. Hanson, L. Reshef, M. F. Hopgood, F. J. Ballard

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

The rate of synthesis of phosphoenolpyruvate carboxykinase (EC 4.1.1.32) in rat liver decreased with a half life of 30 min after fasted rats were re fed either a chow diet or glucose. A requirement for both glucose and insulin to bring about this rapid deinduction was shown, as well as the ability of dibutyryl adenosine 3':5' cyclic monophosphate to block the decrease in enzyme synthesis. Estimates of the stability of messenger RNA of phosphoenolpyruvate carboxykinase were made by using the inhibitors actinomycin D and cordycepin to block further messenger RNA synthesis, and then measuring the decrease in specific enzyme synthesis. It is suggested that the use of actinomycin D yields an overestimation of the template stability. The results with cordycepin imply that the enzyme messenger RNA has a short half life of approximately 1 hr. Thus, it is possible that deinduction may proceed by way of a decrease in messenger RNA production, leading to a rapid fall in the synthesis of phosphoenolpyruvate carboxykinase.

Original languageEnglish (US)
Pages (from-to)1304-1308
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number4
DOIs
StatePublished - 1974

All Science Journal Classification (ASJC) codes

  • General

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