Rap1p telomere association is not required for mitotic stability of a C3TA2 telomere in yeast

Mary Kate Alexander, Virginia A. Zakian

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Telomeric DNA usually consists of a repetitive sequence: C1-3A/TG1-3 in yeast, and C3TA2/T2AG3 in vertebrates. In yeast, the sequence-specific DNA-binding protein Rap1p is thought to be essential for telomere function. In a tlc1h mutant, the templating region of the telomerase RNA gene is altered so that telomerase adds the vertebrate telomere sequence instead of the yeast sequence to the chromosome end. A tlc1h strain has short but stable telomeres and no growth defect. We show here that Rap1p and the Rap1p-associated Rif2p did not bind to a telomere that contains purely vertebrate repeats, while the TG1-3 single-stranded DNA binding protein Cdc13p and the normally non-telomeric protein Tbf1p did bind this telomere. A chromosome with one entirely vertebrate-sequence telomere had a wild-type loss rate, and the telomere was maintained at a short but stable length. However, this telomere was unable to silence a telomere-adjacent URA3 gene, and the strain carrying this telomere had a severe defect in meiosis. We conclude that Rap1p localization to a C3TA2 telomere is not required for its essential mitotic functions.

Original languageEnglish (US)
Pages (from-to)1688-1696
Number of pages9
JournalEMBO Journal
Volume22
Issue number7
DOIs
StatePublished - Apr 1 2003

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Keywords

  • RAP1
  • RIF2
  • Telomerase
  • Telomere
  • Yeast

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