Random conical tilt reconstruction without particle picking in cryo-electron microscopy

Ti Yen Lan, Nicolas Boumal, Amit Singer

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


A method is proposed to reconstruct the 3D molecular structure from micrographs collected at just one sample tilt angle in the random conical tilt scheme in cryo-electron microscopy. The method uses autocorrelation analysis on the micrographs to estimate features of the molecule which are invariant under certain nuisance parameters such as the positions of molecular projections in the micrographs. This enables the molecular structure to be reconstructed directly from micrographs, completely circumventing the need for particle picking. Reconstructions are demonstrated with simulated data and the effect of the missing-cone region is investigated. These results show promise to reduce the size limit for single-particle reconstruction in cryo-electron microscopy.

Original languageEnglish (US)
Pages (from-to)294-301
Number of pages8
JournalActa Crystallographica Section A: Foundations and Advances
StatePublished - Jul 1 2022

All Science Journal Classification (ASJC) codes

  • Condensed Matter Physics
  • General Materials Science
  • Structural Biology
  • Biochemistry
  • Inorganic Chemistry
  • Physical and Theoretical Chemistry


  • autocorrelation analysis
  • cryo-EM
  • random conical tilt
  • structure reconstruction


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