Radical SAM Enzyme QmpB Installs Two 9-Membered Ring Sactionine Macrocycles during Biogenesis of a Ribosomal Peptide Natural Product

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Abstract

We report the reaction catalyzed by QmpB, a new radical S-adenosylmethionine enzyme encoded by a ribosomal peptide natural product gene cluster in Streptococcus suis. Using isotopic labeling, site-directed mutagenesis, high-resolution mass spectrometry, and multidimensional NMR spectroscopy, we show that QmpB installs two 9-membered ring sactionine bridges, connecting a Cys residue with an upstream Asn via an α-thioether bridge, with the two macrocycles separated by a single residue. QmpB is only the second type II sactionine synthase characterized to date.

Original languageEnglish (US)
Pages (from-to)11284-11289
Number of pages6
JournalJournal of Organic Chemistry
Volume86
Issue number16
DOIs
StatePublished - Aug 20 2021

All Science Journal Classification (ASJC) codes

  • Organic Chemistry

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