Quorum sensing regulates the osmotic stress response in Vibrio harveyi

Julia C. van Kessel, Steven T. Rutherford, Jian Ping Cong, Sofia Quinodoz, James Healy, Bonnie L. Basslera

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Bacteria use a chemical communication process called quorum sensing to monitor cell density and to alter behavior in response to fluctuations in population numbers. Previous studies with Vibrio harveyi have shown that LuxR, the master quorum-sensing regulator, activates and represses >600 genes. These include six genes that encode homologs of the Escherichia coli Bet and ProU systems for synthesis and transport, respectively, of glycine betaine, an osmoprotectant used during osmotic stress. Here we show that LuxR activates expression of the glycine betaine operon betIBA-proXWV, which enhances growth recovery under osmotic stress conditions. BetI, an autorepressor of the V. harveyi betIBA-proXWV operon, activates the expression of genes encoding regulatory small RNAs that control quorum-sensing transitions. Connecting quorum-sensing and glycine betaine pathways presumably enables V. harveyi to tune its execution of collective behaviors to its tolerance to stress.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalJournal of bacteriology
Volume197
Issue number1
DOIs
StatePublished - 2015

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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    van Kessel, J. C., Rutherford, S. T., Cong, J. P., Quinodoz, S., Healy, J., & Basslera, B. L. (2015). Quorum sensing regulates the osmotic stress response in Vibrio harveyi. Journal of bacteriology, 197(1), 73-80. https://doi.org/10.1128/JB.02246-14