Quorum sensing in streptococcus mutans regulates production of tryglysin, a novel ras-ripp antimicrobial compound

Britta E. Rued, Brett C. Covington, Leah B. Bushin, Gabriella Szewczyk, Irina Laczkovich, Mohammad R. Seyedsayamdost, Michael J. Federle

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


The genus Streptococcus encompasses a large bacterial taxon that commonly colonizes mucosal surfaces of vertebrates and is capable of disease etiologies originating from diverse body sites, including the respiratory, digestive, and reproductive tracts. Identifying new modes of treating infections is of increasing importance, as antibiotic resistance has escalated. Streptococcus mutans is an important opportunistic pathogen that is an agent of dental caries and is capable of systemic diseases such as endocarditis. As such, understanding how it regulates virulence and competes in the oral niche is a priority in developing strategies to defend from these pathogens. We determined that S. mutans UA159 possesses a bona fide short hydrophobic peptide (SHP)/Rgg quorum-sensing system that regulates a specialized biosynthetic operon featuring a radical-SAM (S-adenosyl-L-methionine) (RaS) enzyme and produces a ribosomally synthesized and posttranslationally modified peptide (RiPP). The pairing of SHP/Rgg regulatory systems with RaS biosynthetic operons is conserved across streptococci, and a locus similar to that in S. mutans is found in Streptococcus ferus, an oral streptococcus isolated from wild rats. We identified the RaS-RiPP product from this operon and solved its structure using a combination of analytical methods; we term these RiPPs tryglysin A and B for the unusual Trp-GlyLys linkage. We report that tryglysins specifically inhibit the growth of other streptococci, but not other Gram-positive bacteria such as Enterococcus faecalis or Lactococcus lactis. We predict that tryglysin is produced by S. mutans in its oral niche, thus inhibiting the growth of competing species, including several medically relevant streptococci. IMPORTANCE Bacteria interact and compete with a large community of organisms in their natural environment. Streptococcus mutans is one such organism, and it is an important member of the oral microbiota. We found that S. mutans uses a quorumsensing system to regulate production of a novel posttranslationally modified peptide capable of inhibiting growth of several streptococcal species. We find inhibitory properties of a similar peptide produced by S. ferus and predict that these peptides play a role in interspecies competition in the oral niche.

Original languageEnglish (US)
Article numbere02688-20
Pages (from-to)1-25
Number of pages25
Issue number2
StatePublished - Mar 1 2021

All Science Journal Classification (ASJC) codes

  • Virology
  • Microbiology


  • Antimicrobial peptides
  • Bacteriocins
  • Quorum sensing
  • Streptococcus


Dive into the research topics of 'Quorum sensing in streptococcus mutans regulates production of tryglysin, a novel ras-ripp antimicrobial compound'. Together they form a unique fingerprint.

Cite this