Quorum sensing controls biofilm formation in Vibrio cholerae

Brian K. Hammer, Bonnie Lynn Bassler

Research output: Contribution to journalArticle

565 Scopus citations

Abstract

Multiple quorum-sensing circuits function in parallel to control virulence and biofilm formation in Vibrio cholerae. In contrast to other bacterial pathogens that induce virulence factor production and/or biofilm formation at high cell density in the presence of quorum-sensing autoinducers, V. cholerae represses these behaviours at high cell density. Consistent with this, we show here that V. cholerae strains 'locked' in the regulatory state mimicking low cell density are enhanced for biofilm production whereas mutants 'locked' in the regulatory state mimicking high cell density are incapable of producing biofilms. The quorum-sensing cascade we have identified in V. cholerae regulates the transcription of genes involved in exopolysaccharide production (EPS), and variants that produce EPS and form biofilms arise at high frequency from non-EPS, non-biofilm producing strains. Our data show that spontaneous mutation of the transcriptional regulator hapR is responsible for this effect. Several toxigenic strains of V. cholerae possess a naturally occurring frameshift mutation in hapR. Thus, the distinct environments occupied by this aquatic pathogen presumably include niches where cell-cell communication is crucial, as well as ones where loss of quorum sensing via hapR mutation confers a selective advantage. Bacterial biofilms could represent a complex habitat where such differentiation occurs.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalMolecular Microbiology
Volume50
Issue number1
DOIs
StatePublished - Oct 2003

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Quorum sensing controls biofilm formation in Vibrio cholerae'. Together they form a unique fingerprint.

  • Cite this