Quantitative proteome dynamics across embryogenesis in a model chordate

Alexander N. Frese, Andrea Mariossi, Michael S. Levine, Martin Wühr

Research output: Contribution to journalArticlepeer-review


The evolution of gene expression programs underlying the development of vertebrates remains poorly characterized. Here, we present a comprehensive proteome atlas of the model chordate Ciona, covering eight developmental stages and ∼7,000 translated genes, accompanied by a multi-omics analysis of co-evolution with the vertebrate Xenopus. Quantitative proteome comparisons argue against the widely held hourglass model, based solely on transcriptomic profiles, whereby peak conservation is observed during mid-developmental stages. Our analysis reveals maximal divergence at these stages, particularly gastrulation and neurulation. Together, our work provides a valuable resource for evaluating conservation and divergence of multi-omics profiles underlying the diversification of vertebrates.

Original languageEnglish (US)
Article number109355
Issue number4
StatePublished - Apr 19 2024

All Science Journal Classification (ASJC) codes

  • General


  • Animals
  • Embryology
  • Evolutionary developmental biology
  • Proteomics
  • Transcriptomics


Dive into the research topics of 'Quantitative proteome dynamics across embryogenesis in a model chordate'. Together they form a unique fingerprint.

Cite this