Quantitative determination of urea concentrations in cell culture medium

Robert J.X. Zawada; Peggy Kwan; Kellen L. Olszewski; Manuel Llinas; Shu Gui Huang

doi:10.1139/O09-011

Quantitative determination of urea concentrations in cell culture medium

Robert J.X. Zawada, Peggy Kwan, Kellen L. Olszewski, Manuel Llinas, Shu Gui Huang

Princeton Branch of the Ludwig Institute for Cancer Research

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.

Original language	English (US)
Pages (from-to)	541-544
Number of pages	4
Journal	Biochemistry and Cell Biology
Volume	87
Issue number	3
DOIs	https://doi.org/10.1139/O09-011
State	Published - Jun 2009

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

Keywords

Cellular urea
Urea assay
Urea determination
Urea metabolism
Urea synthesis

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10.1139/O09-011

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abstract = "Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.",

keywords = "Cellular urea, Urea assay, Urea determination, Urea metabolism, Urea synthesis",

author = "Zawada, \{Robert J.X.\} and Peggy Kwan and Olszewski, \{Kellen L.\} and Manuel Llinas and Huang, \{Shu Gui\}",

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AU - Zawada, Robert J.X.

AU - Kwan, Peggy

AU - Olszewski, Kellen L.

AU - Llinas, Manuel

AU - Huang, Shu Gui

PY - 2009/6

Y1 - 2009/6

N2 - Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.

AB - Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.

KW - Cellular urea

KW - Urea assay

KW - Urea determination

KW - Urea metabolism

KW - Urea synthesis

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JO - Biochemistry and Cell Biology

JF - Biochemistry and Cell Biology

IS - 3

ER -

Quantitative determination of urea concentrations in cell culture medium

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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