Quantifying nitric oxide flux distributions

Darshan M. Sivaloganathan, Xuanqing Wan, Mark P. Brynildsen

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Nitric oxide (NO) is a radical that is used as an attack molecule by immune cells. NO can interact and damage a range of biomolecules, and the biological outcome for bacteria assaulted with NO will be governed by how the radical distributes within their biochemical reaction networks. Measurement of those NO fluxes is complicated by the low abundance and transience of many of its reaction products. To overcome this challenge, we use computational modeling to translate measurements of several biochemical species (e.g., NO, O2, NO2) into NO flux distributions. In this chapter, we provide a detailed protocol, which includes experimental measurements and computational modeling, to estimate the NO flux distribution in an Escherichia coli culture. Those fluxes will have uncertainty associated with them and we also discuss how further experiments and modeling can be employed for flux refinement.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages161-188
Number of pages28
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2088
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Escherichia coli
  • Metabolic flux
  • Nitric oxide
  • Nitric oxide dioxygenase
  • Nitric oxide reductase

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    Sivaloganathan, D. M., Wan, X., & Brynildsen, M. P. (2020). Quantifying nitric oxide flux distributions. In Methods in Molecular Biology (pp. 161-188). (Methods in Molecular Biology; Vol. 2088). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0159-4_8