@article{6ad0d8db68094814a068d3f245739d1b,
title = "Quantifying Cyanothece growth under DIC limitation",
abstract = "The photoautotrophic, unicellular N2-fixer, Cyanothece, is a model organism that has been widely used to study photosynthesis regulation, the structure of photosystems, and the temporal segregation of carbon (C) and nitrogen (N) fixation in light and dark phases of the diel cycle. Here, we present a simple quantitative model and experimental data that together, suggest external dissolved inorganic carbon (DIC) concentration as a major limiting factor for Cyanothece growth, due to its high C-storage requirement. Using experimental data from a parallel laboratory study as a basis, we show that after the onset of the light period, DIC was rapidly consumed by photosynthesis, leading to a sharp drop in the rate of photosynthesis and C accumulation. In N2-fixing cultures, high rates of photosynthesis in the morning enabled rapid conversion of DIC to intracellular C storage, hastening DIC consumption to levels that limited further uptake. The N2-fixing condition allows only a small fraction of fixed C for cellular growth since a large fraction was reserved in storage to fuel night-time N2 fixation. Our model provides a framework for resolving DIC limitation in aquatic ecosystem simulations, where DIC as a growth-limiting factor has rarely been considered, and importantly emphasizes the effect of intracellular C allocation on growth rate that varies depending on the growth environment.",
keywords = "Biomass, CO, Carbon, Carbon allocation, Carbon storage, Cellular growth, Computer simulation, Culture, Cyanothece, DIC, Diurnal cycle, Growth limitation, Mathematical model, Nitrate, Nitrogen fixation, Photosynthesis, Quantitative model, Turbidostat",
author = "Keisuke Inomura and Takako Masuda and Meri Eichner and Sophie Rabouille and Tom{\'a}{\v s} Zav{\v r}el and Jan {\v C}erven{\'y} and Marie Vancov{\'a} and G{\'a}bor Bern{\'a}t and Gabrielle Armin and Pascal Claquin and Eva Kotabov{\'a} and Susanne Stephan and Suggett, {David J.} and Curtis Deutsch and Ond{\v r}ej Pr{\'a}{\v s}il",
note = "Funding Information: We thank Steven G. Ball for sharing useful insights about C storage in Cyanothece, Jos? Bonomi-Barufi for contributing to data acquisition, Douglas A. Campbell for providing feedback, Martin Luke? for his help in measurements and interpretation of data. This project was supported by the Simons Foundation (Simons Postdoctoral Fellowship in Marine Microbial Ecology, Award 544338, KI), the National Science Foundation under EPSCoR Cooperative Agreement (Award OIA-1655221, KI, GA), the Czech Research Foundation GA?R (Award 20-17627S, OP, TM and Award 18-24397S, TZ, J?), MEYS CR (LM2018129 Czech-BioImaging, MV), the Ministry of Education, Youth and Sports of the Czech Republic (OP RDE grant number CZ.02.1.01/0.0/0.0/16?588 026/0008413, TZ, J?), and the National Research, Development and Innovation Office of Hungary, NKFIH, (Award K 128950 and NKFIH-471-3/2021, GB). We thank the generous support from these foundations. Funding Information: We thank Steven G. Ball for sharing useful insights about C storage in Cyanothece, Jos{\'e} Bonomi-Barufi for contributing to data acquisition, Douglas A. Campbell for providing feedback, Martin Luke{\v s} for his help in measurements and interpretation of data. This project was supported by the Simons Foundation (Simons Postdoctoral Fellowship in Marine Microbial Ecology, Award 544338, KI), the National Science Foundation under EPSCoR Cooperative Agreement (Award OIA-1655221, KI, GA), the Czech Research Foundation GA{\v C}R (Award 20-17627S, OP, TM and Award 18-24397S, TZ, J{\v C}), MEYS CR (LM2018129 Czech-BioImaging, MV), the Ministry of Education, Youth and Sports of the Czech Republic (OP RDE grant number CZ.02.1.01/0.0/0.0/16–588 026/0008413, TZ, J{\v C}), and the National Research, Development and Innovation Office of Hungary, NKFIH, (Award K 128950 and NKFIH-471-3/2021, GB). We thank the generous support from these foundations. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jan,
doi = "10.1016/j.csbj.2021.11.036",
language = "English (US)",
volume = "19",
pages = "6456--6464",
journal = "Computational and Structural Biotechnology Journal",
issn = "2001-0370",
publisher = "Research Network of Computational and Structural Biotechnology",
}