In nongrowing microbes, proteome turnover is reduced and identification of newly synthesized, low-abundance proteins is challenging. Babin and colleagues recently utilized bio-orthogonal noncanonical amino acid tagging (BONCAT) to identify actively synthesized proteins in nongrowing Pseudomonas aeruginosa, discovering a regulator whose influences range from biofilm formation to secondary metabolism.
All Science Journal Classification (ASJC) codes
- Microbiology (medical)
- Infectious Diseases
- Nongrowing cells
- Pseudomonas aeruginosa