Quantification of catechol 2,3-dioxygenase gene homology and benzoate utilization in intertidal sediments

Chris A. Francis, Alisa Kirk Francis, Deborah S. Golet, Bettie Ward

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The catechol 2,3-dioxygenase (C23O) gene, xylE, from the TOL plasmid pathway was used to probe naturally occurring bacterial communities in an intertidal microbial mat. Bound probe was quantified by densitometric analysis of slot blots using colorimetric detection of hybridization between the probe and total DNA extracts from the sediments. The C23O gene encodes the key ring-breaking enzyme of the toluene degradation pathway, of which benzoate is an intermediate. Radiotracer experiments using 14C-benzoate detected benzoate mineralization in these sediments, corroborating the presence of both the genetic potential and in situ activity for this transformation.

Original languageEnglish (US)
Pages (from-to)225-231
Number of pages7
JournalAquatic Microbial Ecology
Volume15
Issue number3
DOIs
StatePublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Keywords

  • Benzoate utilization
  • Catechol 2,3-dioxygenase
  • Microbial mats
  • Quantitative hybridization

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