Protocol for transgenerational learned pathogen avoidance behavior assays in Caenorhabditis elegans

Rebecca S. Moore, Rachel Kaletsky, Coleen T. Murphy

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Animal experiences, including learned behaviors, can be passed down to several generations of progeny in a phenomenon known as transgenerational epigenetic inheritance. Yet, little is known regarding the molecular mechanisms regulating physiologically relevant transgenerational memories. Here, we present a method for Caenorhabditis elegans in which worms learn to avoid the pathogen Pseudomonas aeruginosa (PA14). Unlike previous protocols, this training paradigm, either using PA14 lawns or through exposure to a PA14 small RNA (P11), induces memory in four generations of progeny. For complete details on the use and execution of this protocol, please refer to Moore et al. (2019) and Kaletsky et al. (2020).

Original languageEnglish (US)
Pages (from-to)100384
Number of pages1
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Neuroscience

Keywords

  • Genetics
  • Model organisms

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