Protocol for the isolation, culture, and transfection of squid primary cells

Yoonji Kim; Hailey M. Tanner; Joshua J.C. Rosenthal; Clifford P. Brangwynne

doi:10.1016/j.xpro.2025.103994

Protocol for the isolation, culture, and transfection of squid primary cells

Yoonji Kim, Hailey M. Tanner, Joshua J.C. Rosenthal, Clifford P. Brangwynne

Research output: Contribution to journal › Article › peer-review

Abstract

We present a protocol for the isolation, culture, and transfection of squid primary cells from various tissues and ages by including steps for squid dissection, cell isolation, maintenance in culture, and expression of exogenous genes. We also highlight life-stage tractability in Euprymna berryi squids for future aging studies. This protocol enables molecular- and cellular-level investigations into processes specific to squids, including their complex behaviors, rapid color change mechanisms, and RNA editing capabilities, with broader implications in basic cell physiology.

Original language	English (US)
Article number	103994
Journal	STAR Protocols
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2025.103994
State	Published - Sep 19 2025

All Science Journal Classification (ASJC) codes

General Neuroscience
General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology

Keywords

cell biology
model organisms

Access to Document

10.1016/j.xpro.2025.103994

Cite this

@article{80c7bb178337454bb071d74932ef885c,

title = "Protocol for the isolation, culture, and transfection of squid primary cells",

abstract = "We present a protocol for the isolation, culture, and transfection of squid primary cells from various tissues and ages by including steps for squid dissection, cell isolation, maintenance in culture, and expression of exogenous genes. We also highlight life-stage tractability in Euprymna berryi squids for future aging studies. This protocol enables molecular- and cellular-level investigations into processes specific to squids, including their complex behaviors, rapid color change mechanisms, and RNA editing capabilities, with broader implications in basic cell physiology.",

keywords = "cell biology, model organisms",

author = "Yoonji Kim and Tanner, \{Hailey M.\} and Rosenthal, \{Joshua J.C.\} and Brangwynne, \{Clifford P.\}",

note = "Publisher Copyright: {\textcopyright} 2025",

year = "2025",

month = sep,

day = "19",

doi = "10.1016/j.xpro.2025.103994",

language = "English (US)",

volume = "6",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "3",

}

TY - JOUR

T1 - Protocol for the isolation, culture, and transfection of squid primary cells

AU - Kim, Yoonji

AU - Tanner, Hailey M.

AU - Rosenthal, Joshua J.C.

AU - Brangwynne, Clifford P.

PY - 2025/9/19

Y1 - 2025/9/19

N2 - We present a protocol for the isolation, culture, and transfection of squid primary cells from various tissues and ages by including steps for squid dissection, cell isolation, maintenance in culture, and expression of exogenous genes. We also highlight life-stage tractability in Euprymna berryi squids for future aging studies. This protocol enables molecular- and cellular-level investigations into processes specific to squids, including their complex behaviors, rapid color change mechanisms, and RNA editing capabilities, with broader implications in basic cell physiology.

AB - We present a protocol for the isolation, culture, and transfection of squid primary cells from various tissues and ages by including steps for squid dissection, cell isolation, maintenance in culture, and expression of exogenous genes. We also highlight life-stage tractability in Euprymna berryi squids for future aging studies. This protocol enables molecular- and cellular-level investigations into processes specific to squids, including their complex behaviors, rapid color change mechanisms, and RNA editing capabilities, with broader implications in basic cell physiology.

KW - cell biology

KW - model organisms

UR - https://www.scopus.com/pages/publications/105012554414

UR - https://www.scopus.com/inward/citedby.url?scp=105012554414&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2025.103994

DO - 10.1016/j.xpro.2025.103994

M3 - Article

C2 - 40773350

AN - SCOPUS:105012554414

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 3

M1 - 103994

ER -

Protocol for the isolation, culture, and transfection of squid primary cells

Abstract

All Science Journal Classification (ASJC) codes

Keywords

Access to Document

Other files and links

Fingerprint

Cite this