Proteotoxicity from aberrant ribosome biogenesis compromises cell fitness

Blake W. Tye, Nicoletta Commins, Lillia V. Ryazanova, Martin Wühr, Michael Springer, David Pincus, L. Stirling Churchman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

To achieve maximal growth, cells must manage a massive economy of ribosomal proteins (r-proteins) and RNAs (rRNAs) to produce thousands of ribosomes every minute. Although ribosomes are essential in all cells, natural disruptions to ribosome biogenesis lead to heterogeneous phenotypes. Here, we model these perturbations in Saccharomyces cerevisiae and show that challenges to ribosome biogenesis result in acute loss of proteostasis. Imbalances in the synthesis of r-proteins and rRNAs lead to the rapid aggregation of newly synthesized orphan r-proteins and compromise essential cellular processes, which cells alleviate by activating proteostasis genes. Exogenously bolstering the proteostasis network increases cellular fitness in the face of challenges to ribosome assembly, demonstrating the direct contribution of orphan r-proteins to cellular phenotypes. We propose that ribosome assembly is a key vulnerability of proteostasis maintenance in proliferating cells that may be compromised by diverse genetic, environmental, and xenobiotic perturbations that generate orphan r-proteins.

Original languageEnglish (US)
Article numbere43002
JournaleLife
Volume8
DOIs
StatePublished - Mar 2019

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Tye, B. W., Commins, N., Ryazanova, L. V., Wühr, M., Springer, M., Pincus, D., & Churchman, L. S. (2019). Proteotoxicity from aberrant ribosome biogenesis compromises cell fitness. eLife, 8, [e43002]. https://doi.org/10.7554/eLife.43002