Protein stapling via azide–alkyne ligation

Diya M. Abdeljabbar; Frank J. Piscotta; Siyan Zhang; A. James Link

doi:10.1039/c4cc06528j

Protein stapling via azide–alkyne ligation

Diya M. Abdeljabbar, Frank J. Piscotta, Siyan Zhang, A. James Link

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.

Original language	English (US)
Pages (from-to)	14900-14903
Number of pages	4
Journal	Chemical Communications
Volume	50
Issue number	94
DOIs	https://doi.org/10.1039/c4cc06528j
State	Published - Oct 30 2014

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

Access to Document

10.1039/c4cc06528j

Cite this

@article{aff8d84e80c64e70ba0dbcb5e6749b05,

title = "Protein stapling via azide–alkyne ligation",

abstract = "Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.",

author = "Abdeljabbar, {Diya M.} and Piscotta, {Frank J.} and Siyan Zhang and Link, {A. James}",

year = "2014",

month = oct,

day = "30",

doi = "10.1039/c4cc06528j",

language = "English (US)",

volume = "50",

pages = "14900--14903",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "94",

}

TY - JOUR

T1 - Protein stapling via azide–alkyne ligation

AU - Abdeljabbar, Diya M.

AU - Piscotta, Frank J.

AU - Zhang, Siyan

AU - Link, A. James

PY - 2014/10/30

Y1 - 2014/10/30

N2 - Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.

AB - Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.

UR - http://www.scopus.com/inward/record.url?scp=84908637095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908637095&partnerID=8YFLogxK

U2 - 10.1039/c4cc06528j

DO - 10.1039/c4cc06528j

M3 - Article

C2 - 25325394

AN - SCOPUS:84908637095

SN - 1359-7345

VL - 50

SP - 14900

EP - 14903

JO - Chemical Communications

JF - Chemical Communications

IS - 94

ER -

Protein stapling via azide–alkyne ligation

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this