Protein stapling via azide–alkyne ligation

Diya M. Abdeljabbar, Frank J. Piscotta, Siyan Zhang, A. James Link

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Here we demonstrate a methodology, termed protein stapling, for the introduction of covalent constraints into recombinant proteins. Using the azide–alkyne click reaction as the stapling chemistry, we have improved the thermostability of a model leucine zipper protein. Additionally, stapling the core of the small, globular protein G resulted in improved binding to its target, immunoglobulin G.

Original languageEnglish (US)
Pages (from-to)14900-14903
Number of pages4
JournalChemical Communications
Issue number94
StatePublished - Oct 30 2014

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • General Chemistry
  • Ceramics and Composites
  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Catalysis


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