TY - JOUR
T1 - Properties of the transcriptional enhancer in Saccharomyces cerevisiae telomeres
AU - Runge, Kurt W.
AU - Zakian, Virginia A.
N1 - Funding Information:
K. R. was supported by N.I.H. postdoctoral fellowship GM10472-03.
Funding Information:
We would like to thank L. Breeden, M. Conrad, L. Sandell, R. Wellinger, and J. Wright for helpfiil discussions on this manuscript. This work was supported by ACS grant NP-574.
PY - 1990/4/11
Y1 - 1990/4/11
N2 - Saccharomyces cerevisiae chromosomes end wtth the sequence C2-3A(CA)1-4, commonly abbreviated as C1-3A. These sequences can function as upstream activators of transcription (UAS's) when placed in front of a CYC1-lacZ fusion gene. When C1-3 sequences are placed between the GAL1,10 UAS and the CYC1-lacZ fusion, the C1-3A UAS still functions and the amount of β-galactosidase produced in cells grown on glucose is as much or more than that for cells grown on either glycerol medium, or cells grown on glucose medium containing a plasmid with just the C1-3A UAS. These data indicate that the UAS is immune from glucose repression from the upstream GAL 1,10 UAS. Because C1-3A sequences are bound in vitro by the transcription factor RAP1, the UAS activity of yeast telomere sequences was compared with that of a similar UAS from the tightly regulated ribosomal protein gene RP39A, which also contains a RAP1 binding site. While transcription from the ribosomal protein gene UAS was responsive to cell density, the amount of transcription from the C1-3A UAS was nearly the same at all cell densities tested. These data show that the transcriptional activation by C1-3A sequences is not regulated by cell density.
AB - Saccharomyces cerevisiae chromosomes end wtth the sequence C2-3A(CA)1-4, commonly abbreviated as C1-3A. These sequences can function as upstream activators of transcription (UAS's) when placed in front of a CYC1-lacZ fusion gene. When C1-3 sequences are placed between the GAL1,10 UAS and the CYC1-lacZ fusion, the C1-3A UAS still functions and the amount of β-galactosidase produced in cells grown on glucose is as much or more than that for cells grown on either glycerol medium, or cells grown on glucose medium containing a plasmid with just the C1-3A UAS. These data indicate that the UAS is immune from glucose repression from the upstream GAL 1,10 UAS. Because C1-3A sequences are bound in vitro by the transcription factor RAP1, the UAS activity of yeast telomere sequences was compared with that of a similar UAS from the tightly regulated ribosomal protein gene RP39A, which also contains a RAP1 binding site. While transcription from the ribosomal protein gene UAS was responsive to cell density, the amount of transcription from the C1-3A UAS was nearly the same at all cell densities tested. These data show that the transcriptional activation by C1-3A sequences is not regulated by cell density.
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U2 - 10.1093/nar/18.7.1783
DO - 10.1093/nar/18.7.1783
M3 - Article
C2 - 2110655
AN - SCOPUS:0025274477
SN - 0305-1048
VL - 18
SP - 1783
EP - 1787
JO - Nucleic acids research
JF - Nucleic acids research
IS - 7
ER -