The theoretical basis of new methods for studying single-molecule dynamics over the time scales of enzymatic importance is presented. As such, a general methodology that is based on fluctuation-correlation analysis of experimental observables photon by photon is introduced. This statistically efficient method not only extends the fast measurable time scale to a microsecond or less, but also allows the education of information from the limited number of detected photons of a single molecule over the time scales that are important in characterizing the function of a biological macromolecule.
All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)
- Physical and Theoretical Chemistry