Abstract
The theoretical basis of new methods for studying single-molecule dynamics over the time scales of enzymatic importance is presented. As such, a general methodology that is based on fluctuation-correlation analysis of experimental observables photon by photon is introduced. This statistically efficient method not only extends the fast measurable time scale to a microsecond or less, but also allows the education of information from the limited number of detected photons of a single molecule over the time scales that are important in characterizing the function of a biological macromolecule.
Original language | English (US) |
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Pages (from-to) | 10965-10979 |
Number of pages | 15 |
Journal | Journal of Chemical Physics |
Volume | 117 |
Issue number | 24 |
DOIs | |
State | Published - Dec 22 2002 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- General Physics and Astronomy
- Physical and Theoretical Chemistry