Presentation and detection of azide functionality in bacterial cell surface proteins

A. James Link, Mandy K.S. Vink, David A. Tirrell

Research output: Contribution to journalArticlepeer-review

292 Scopus citations


An improved protocol for copper-catalyzed triazole formation on the bacterial cell surface is described. Addition of highly pure CuBr to cells treated with azidohomoalanine (2) leads to ca. 10-fold more extensive cell surface labeling than previously observed. This highly active catalyst allows detection of the methionine analogues azidoalanine (1), azidonorvaline (3), and azidonorleucine (4) in cell surface proteins. Azidoalanine was previously believed to be silent with regard to the cellular protein synthesis machinery.

Original languageEnglish (US)
Pages (from-to)10598-10602
Number of pages5
JournalJournal of the American Chemical Society
Issue number34
StatePublished - Sep 1 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry


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