Preparing, handling, and applying caged compound solutions for acousto-optical deflector-based patterned uncaging with ultraviolet light

Eugene F. Civillico, Shy Shoham, Daniel H. O'Connor, Dmitry V. Sarkisov, Samuel S.H. Wang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The method of patterned photoactivation is a natural fit for the study of neuronal dendritic integration. Photoactivatable molecules that influence a wide range of extracellular and intracellular neurophysiological functions are available. The choice of photosensitive molecules depends on the research question and will influence the design of the experimental apparatus. An acousto-optical deflector (AOD)-based system can be used for rapid ultraviolet (UV) photolysis in arbitrary spatial and temporal patterns. This protocol describes how to prepare caged neurotransmitter compound solutions for use in this system and discusses options for introducing caged compounds into an experimental preparation.

Original languageEnglish (US)
Pages (from-to)923-925
Number of pages3
JournalCold Spring Harbor Protocols
Volume7
Issue number8
DOIs
StatePublished - Aug 2012

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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