Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging

Evan W. Miller; Qiwen He; Christopher J. Chang

doi:10.1038/nprot.2008.43

Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging

Evan W. Miller, Qiwen He, Christopher J. Chang

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Leadfluor-1 (LF1) is a small-molecule fluorescent sensor for detecting lead in biological and environmental samples, including live cells. This dye uses a xanthenone fluorescent scaffold coupled to a dicarboxylate pseudocrown ether receptor to achieve selective detection of Pb²⁺ in the presence of biologically relevant metal ions, including divalent calcium, magnesium and zinc. LF1 fluorescence increases by up to 18-fold on binding Pb²⁺. In this protocol, we describe the synthesis and application of LF1 to imaging lead accumulation within live cells. The preparation of LF1 is anticipated to take 14-21 d, and the imaging assays can be performed in 1-2 d with cultured cells.

Original language	English (US)
Pages (from-to)	777-783
Number of pages	7
Journal	Nature Protocols
Volume	3
Issue number	5
DOIs	https://doi.org/10.1038/nprot.2008.43
State	Published - Apr 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1038/nprot.2008.43

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abstract = "Leadfluor-1 (LF1) is a small-molecule fluorescent sensor for detecting lead in biological and environmental samples, including live cells. This dye uses a xanthenone fluorescent scaffold coupled to a dicarboxylate pseudocrown ether receptor to achieve selective detection of Pb2+ in the presence of biologically relevant metal ions, including divalent calcium, magnesium and zinc. LF1 fluorescence increases by up to 18-fold on binding Pb2+. In this protocol, we describe the synthesis and application of LF1 to imaging lead accumulation within live cells. The preparation of LF1 is anticipated to take 14-21 d, and the imaging assays can be performed in 1-2 d with cultured cells.",

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AB - Leadfluor-1 (LF1) is a small-molecule fluorescent sensor for detecting lead in biological and environmental samples, including live cells. This dye uses a xanthenone fluorescent scaffold coupled to a dicarboxylate pseudocrown ether receptor to achieve selective detection of Pb2+ in the presence of biologically relevant metal ions, including divalent calcium, magnesium and zinc. LF1 fluorescence increases by up to 18-fold on binding Pb2+. In this protocol, we describe the synthesis and application of LF1 to imaging lead accumulation within live cells. The preparation of LF1 is anticipated to take 14-21 d, and the imaging assays can be performed in 1-2 d with cultured cells.

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Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging

Abstract

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