Preferential loading of Bergmann glia with synthetic acetoxymethyl calcium dyes

Tycho M. Hoogland, Bernd Kuhn, Samuel S.H. Wang

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The cerebellar cortex contains two astrocyte types: the Bergmann glia of the molecular layer and the velate protoplasmic astrocytes of the granule cell layer. In vivo, these cell types generate both subcellular calcium transients and trans-glial calcium waves. This protocol outlines a method for in vivo calcium imaging in cerebellar astrocytes of mice which have undergone a cerebellar craniotomy. Multicell bolus loading (MCBL) is performed using the synthetic calcium indicators Fluo-5F AM and Fluo-4 AM. In the cerebellum, a degree of cell-type specificity can be achieved by varying the depth of injection. This protocol describes a loading procedure following craniotomy which allows preferential labeling of Bergmann glia.

Original languageEnglish (US)
Pages (from-to)1228-1231
Number of pages4
JournalCold Spring Harbor Protocols
Volume6
Issue number10
DOIs
StatePublished - Oct 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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