@article{314e31caa37243ae8d0c75641ec33c57,
title = "PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism",
abstract = "Metabolic program expression and dependency vary along the length of the small intestine. PRDM16 transcriptionally regulates fatty acid oxidation (FAO), which is highest in the upper small intestine. Loss of Prdm16 reduces FAO, leading to apoptosis and diminished epithelial differentiation of progenitor cells in the upper intestine.",
keywords = "PRDM16, apoptosis, differentiation, duodenum, fatty acid oxidation, intestinal stem cell, intestine, metabolism, transit amplifying cell",
author = "Stine, {Rachel R.} and Sakers, {Alexander P.} and Tara TeSlaa and Megan Kissig and Stine, {Zachary E.} and Kwon, {Chan Wook} and Lan Cheng and Lim, {Hee Woong} and Kaestner, {Klaus H.} and Rabinowitz, {Joshua D.} and Patrick Seale",
note = "Funding Information: We thank members of the Seale laboratory for helpful discussions and careful reading of the manuscript. We thank M. Zhou, R. Fadnavis, and C. Ghougasian for technical assistance and Chi Dang for helpful comments. We also thank the Functional Genomics Core of the Penn Diabetes Center (P30-DK19525) for sequencing, the Human Immunology Core (P30-CA016520) for Luminex analysis, and the UPenn Histology and Gene Expression Core. This work was supported by start-up funds provided by the UPenn Institute for Diabetes , Obesity and Metabolism , NIH grants R01-DK10300802 and R01-DK107589 to P.S., F32-DK10574303 to R.R.S., and a pilot grant from the Center for Molecular Studies in Digestive and Liver Diseases ( NIH P30-DK050306 ). Funding Information: We thank members of the Seale laboratory for helpful discussions and careful reading of the manuscript. We thank M. Zhou, R. Fadnavis, and C. Ghougasian for technical assistance and Chi Dang for helpful comments. We also thank the Functional Genomics Core of the Penn Diabetes Center (P30-DK19525) for sequencing, the Human Immunology Core (P30-CA016520) for Luminex analysis, and the UPenn Histology and Gene Expression Core. This work was supported by start-up funds provided by the UPenn Institute for Diabetes, Obesity and Metabolism, NIH grants R01-DK10300802 and R01-DK107589 to P.S. F32-DK10574303 to R.R.S. and a pilot grant from the Center for Molecular Studies in Digestive and Liver Diseases (NIH P30-DK050306). R.R.S. and P.S. conceived the project and wrote the manuscript, with important input from K.H.K. R.R.S. designed, performed, and interpreted the majority of the experiments. A.P.S. performed RNA-seq analysis and flow-sorting experiments. T.T. performed and analyzed the liquid chromatography-mass spectrometry (LC-MS) tracing experiments with input from J.D.R. C.W.K. participated in enteroid experiments and characterization of mouse models. M.K. participated in glucose-tracing experiments, ChIP, and crypt isolation for RNA and western blot analysis. H.-W.L. performed ChIP-seq analysis. L.C. performed histological and immunofluorescence studies. Z.E.S. assisted in the conception and execution of metabolic tracing experiments, manuscript writing, and data interpretation. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = dec,
day = "5",
doi = "10.1016/j.stem.2019.08.017",
language = "English (US)",
volume = "25",
pages = "830--845.e8",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "6",
}