Position- and hippo signaling-dependent plasticity during lineage segregation in the early mouse embryo

Eszter Posfai, Sophie Petropoulos, Flavia Regina Oliveira de Barros, John Paul Schell, Igor Jurisica, Rickard Sandberg, Fredrik Lanner, Janet Rossant

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

The segregation of the trophectoderm (TE) from the inner cell mass (ICM) in the mouse blastocyst is determined by position-dependent Hippo signaling. However, the window of responsiveness to Hippo signaling, the exact timing of lineage commitment and the overall relationship between cell commitment and global gene expression changes are still unclear. Single- cell RNA sequencing during lineage segregation revealed that the TE transcriptional profile stabilizes earlier than the ICM and prior to blastocyst formation. Using quantitative Cdx2-eGFP expression as a readout of Hippo signaling activity, we assessed the experimental potential of individual blastomeres based on their level of Cdx2-eGFP expression and correlated potential with gene expression dynamics. We find that TE specification and commitment coincide and occur at the time of transcriptional stabilization, whereas ICM cells still retain the ability to regenerate TE up to the early blastocyst stage. Plasticity of both lineages is coincident with their window of sensitivity to Hippo signaling.

Original languageEnglish (US)
Article numbere22906
JournaleLife
Volume6
DOIs
StatePublished - Feb 22 2017

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Posfai, E., Petropoulos, S., de Barros, F. R. O., Schell, J. P., Jurisica, I., Sandberg, R., Lanner, F., & Rossant, J. (2017). Position- and hippo signaling-dependent plasticity during lineage segregation in the early mouse embryo. eLife, 6, [e22906]. https://doi.org/10.7554/eLife.22906