TY - GEN
T1 - Polymer-protected liposomes
T2 - Association of hydrophobically-modified PEG with liposomes
AU - Auguste, Debra T.
AU - Prud'homme, Robert K.
AU - Ahl, Patrick L.
AU - Meers, Paul
AU - Kohn, Joachim
PY - 2006
Y1 - 2006
N2 - We demonstrate the impact of cooperative interactions on liposome protection by incorporating multiple hydrophobic sites on poly(ethylene glycol) (PEG) polymers. Our hydrophobically-modified PEGs (HMPEGs) are comb-graft copolymers with precisely alternating, monodisperse PEG blocks (MW= 6, 12, or 35 kDa), bonded to C18 stearylamide hydrophobes. Cooperativity is controlled by varying the extent of oligomerization at a constant ratio of PEG to stearlyamide. The association of polymer with liposomes increases with the degree of oligomerization; equilibrium constants for 6 kDa PEG increases from 6.1±0.8 (mg/m2)/(mg/ml) for 3 loops to 78.1112.2 (mg/m 2)/(mg/ml) for 13 loops. In addition, HMPEG6k-DP3 (with three 6 kDa loops) results in superior, irreversible protection from complement protein binding than DSPE-PEG5k after 12 hours.
AB - We demonstrate the impact of cooperative interactions on liposome protection by incorporating multiple hydrophobic sites on poly(ethylene glycol) (PEG) polymers. Our hydrophobically-modified PEGs (HMPEGs) are comb-graft copolymers with precisely alternating, monodisperse PEG blocks (MW= 6, 12, or 35 kDa), bonded to C18 stearylamide hydrophobes. Cooperativity is controlled by varying the extent of oligomerization at a constant ratio of PEG to stearlyamide. The association of polymer with liposomes increases with the degree of oligomerization; equilibrium constants for 6 kDa PEG increases from 6.1±0.8 (mg/m2)/(mg/ml) for 3 loops to 78.1112.2 (mg/m 2)/(mg/ml) for 13 loops. In addition, HMPEG6k-DP3 (with three 6 kDa loops) results in superior, irreversible protection from complement protein binding than DSPE-PEG5k after 12 hours.
UR - http://www.scopus.com/inward/record.url?scp=36949003715&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36949003715&partnerID=8YFLogxK
U2 - 10.1021/bk-2006-0923.ch008
DO - 10.1021/bk-2006-0923.ch008
M3 - Conference contribution
AN - SCOPUS:36949003715
SN - 0841239185
SN - 9780841239180
T3 - ACS Symposium Series
SP - 95
EP - 120
BT - Polymeric Drug Delivery I
PB - American Chemical Society
ER -