TY - JOUR
T1 - Polycomb function during oogenesis is required for mouse embryonic development
AU - Posfai, Eszter
AU - Kunzmann, Rico
AU - Brochard, Vincent
AU - Salvaing, Juliette
AU - Cabuy, Erik
AU - Roloff, Tim C.
AU - Liu, Zichuan
AU - Tardat, Mathieu
AU - van Lohuizen, Maarten
AU - Vidal, Miguel
AU - Beaujean, Nathalie
AU - Peters, Antoine H.F.M.
PY - 2012
Y1 - 2012
N2 - In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition of totipotency concurs with chromatin remodeling of parental genomes, changes in the maternal transcriptome and proteome, and zygotic genome activation (ZGA). The inefficiency of reprogramming somatic nuclei in reproductive cloning suggests that intergenerational inheritance of germline chromatin contributes to developmental proficiency after natural conception. Here we show that Ring1 and Rnf2, components of Polycomb-repressive complex 1 (PRC1), serve redundant transcriptional functions during oogenesis that are essential for proper ZGA, replication and cell cycle progression in early embryos, and development beyond the two-cell stage. Exchange of chromosomes between control and Ring1/Rnf2-deficient metaphase II oocytes reveal cytoplasmic and chromosome-based contributions by PRC1 to embryonic development. Our results strongly support a model in which Polycomb acts in the female germline to establish developmental competence for the following generation by silencing differentiation-inducing genes and defining appropriate chromatin states.
AB - In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition of totipotency concurs with chromatin remodeling of parental genomes, changes in the maternal transcriptome and proteome, and zygotic genome activation (ZGA). The inefficiency of reprogramming somatic nuclei in reproductive cloning suggests that intergenerational inheritance of germline chromatin contributes to developmental proficiency after natural conception. Here we show that Ring1 and Rnf2, components of Polycomb-repressive complex 1 (PRC1), serve redundant transcriptional functions during oogenesis that are essential for proper ZGA, replication and cell cycle progression in early embryos, and development beyond the two-cell stage. Exchange of chromosomes between control and Ring1/Rnf2-deficient metaphase II oocytes reveal cytoplasmic and chromosome-based contributions by PRC1 to embryonic development. Our results strongly support a model in which Polycomb acts in the female germline to establish developmental competence for the following generation by silencing differentiation-inducing genes and defining appropriate chromatin states.
KW - Epigenetic memory
KW - Intergenerational inheritance
KW - Intra-S-phase checkpoint
KW - Maternal effect
KW - Nuclear transfer
KW - Polycomb-repressive complex 1
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U2 - 10.1101/gad.188094.112
DO - 10.1101/gad.188094.112
M3 - Article
C2 - 22499591
AN - SCOPUS:84860504565
SN - 0890-9369
VL - 26
SP - 920
EP - 932
JO - Genes and Development
JF - Genes and Development
IS - 9
ER -