@article{30cb645a4de845119620e0d719e28e35,
title = "Pif1 family helicases suppress genome instability at G-quadruplex motifs",
abstract = "The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.",
author = "Katrin Paeschke and Bochman, {Matthew L.} and {Daniela Garcia}, P. and Petr Cejka and Friedman, {Katherine L.} and Kowalczykowski, {Stephen C.} and Zakian, {Virginia A.}",
note = "Funding Information: Acknowledgements We thank J. B. Boule for early work on S. cerevisiae Pif1 biochemistry, P. Opresko for the gift of purified human WRN, E. Allen-Vercoe, K. Bidle, C. Parker, R. Johnson, H. L. Ayala-del-Rio and E. Sockett for materials and for cloning non-yeast Pif1 helicases, and M. Platts for multiplex PCR and Southern analysis methods to characterize GCR clones. We acknowledge financial support from the National Institutes of Health (V.A.Z., S.C.K.), National Science Foundation (K.L.F.), DFG and NJCCR (K.P.) and American Cancer Society (M.L.B.).",
year = "2013",
doi = "10.1038/nature12149",
language = "English (US)",
volume = "497",
pages = "458--462",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Research",
number = "7450",
}