Picosecond thermometer in the amide I band of myoglobin

Robert H. Austin, Aihua Xie, Lex Van Der Meer, Britta Redlich, Per Anker Lindgård, Hans Frauenfelder, Dan Fu

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Abstract

The amide I and II bands in myoglobin show a heterogeneous temperature dependence, with bands at 6.17 and 6.43 μm which are more intense at low temperatures. The amide I band temperature dependence is on the long wavelength edge of the band, while the short wavelength side has almost no temperature dependence. We compare concepts of anharmonic solid-state crystal physics and chemical physics for the origins of these bands. We suggest that the long wavelength side is composed of those amino acids which hydrogen bond to the hydration shell of the protein, and that temperature dependent bands can be used to determine the time it takes vibrational energy to flow into the hydration shell. We determine that vibrational energy flow to the hydration shell from the amide I takes approximately 20 ps to occur.

Original languageEnglish (US)
Article number128101
JournalPhysical review letters
Volume94
Issue number12
DOIs
StatePublished - Apr 1 2005

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

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    Austin, R. H., Xie, A., Van Der Meer, L., Redlich, B., Lindgård, P. A., Frauenfelder, H., & Fu, D. (2005). Picosecond thermometer in the amide I band of myoglobin. Physical review letters, 94(12), [128101]. https://doi.org/10.1103/PhysRevLett.94.128101