TY - JOUR
T1 - Physical map of two D. melanogaster DNA segments containing sequences coding for the 70,000 dalton heat shock protein
AU - Moran, Larry
AU - Mirault, Marc Edouard
AU - Tissières, Alfred
AU - Lis, John
AU - Schedl, Paul
AU - Artavanis-Tsakonas, Spyros
AU - Gehring, Walter J.
N1 - Funding Information:
This work was supported by grants from the Swiss National Science Foundation (to W.J.G. and A.T.). S.A.-T. was supported by a long-term EMBO fellowship and the Swiss National Science Foundation (grant to W.J.G.). P.S. and J.L. were postdoctoral fellows of the Helen Hay Whitney Foundation. P.S. was also supported by the Roche Research Foundation for Scientific Exchange and Biomedical Collaboration with Switzerland. We thank M. Schedl, G. Martin, M. Stamper and M. Stempfel for their valuable assistance; 0. Jenni for drawings and plates; Dr. T. Bickle for a generous gift of restriction enzymes; Dr. David Hogness for his advice: and Dr. Jean-David Rochaix for his comments on the manuscript.
PY - 1979/5
Y1 - 1979/5
N2 - The isolation of the two hybrid plasmids 56H8 and 132E3, which contain D. melanogaster (Dm) DNA sequences complementary to the mRNA coding for the 70,000 dalton heat shock protein, has been reported (Schedl et al., 1978). Here we compare the sequence arrangement in the two cloned Dm DNA segments by restriction, cross-hybridization and heteroduplex analysis. The results show that the two cloned DNA segments derive from nonoverlapping regions of the Dm genome; that they contain homologous regions present once in 56H8 and twice in 132E3; and that each homologous region is composed of three distinct contiguous sequence elements, x, y and z, which together define a 3 kb common unit. While the 2.5 kb z elements show a high degree of sequence homology in all three common units, the three x and y elements display an intriguing relationship. The localization of the mRNA coding sequences within each of these common units is presented in the accompanying paper (Artavanis-Tsakonas et al., 1979).
AB - The isolation of the two hybrid plasmids 56H8 and 132E3, which contain D. melanogaster (Dm) DNA sequences complementary to the mRNA coding for the 70,000 dalton heat shock protein, has been reported (Schedl et al., 1978). Here we compare the sequence arrangement in the two cloned Dm DNA segments by restriction, cross-hybridization and heteroduplex analysis. The results show that the two cloned DNA segments derive from nonoverlapping regions of the Dm genome; that they contain homologous regions present once in 56H8 and twice in 132E3; and that each homologous region is composed of three distinct contiguous sequence elements, x, y and z, which together define a 3 kb common unit. While the 2.5 kb z elements show a high degree of sequence homology in all three common units, the three x and y elements display an intriguing relationship. The localization of the mRNA coding sequences within each of these common units is presented in the accompanying paper (Artavanis-Tsakonas et al., 1979).
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U2 - 10.1016/0092-8674(79)90289-7
DO - 10.1016/0092-8674(79)90289-7
M3 - Article
C2 - 110458
AN - SCOPUS:0018387170
SN - 0092-8674
VL - 17
SP - 1
EP - 8
JO - Cell
JF - Cell
IS - 1
ER -