Physical linkage of two mammalian imprinted genes, H19 and insulin−like growth factor 2

Sharon Zemel, Marisa S. Bartolomei, Shirley Marie Tilghman

Research output: Contribution to journalArticle

239 Scopus citations

Abstract

Parental imprinting is a phenomenon in mammals whereby the maternal and paternal alleles of a gene are differentially expressed. Three murine genes have been shown to display this type of allele−specific expression. Two of them, insulin−like growth factor−2 (Igf−2) and H19, map to the distal end of mouse chromosome 7, but are imprinted in opposite directions. Pulsed−field gel electrophoresis and large−fragment DNA cloning were utilized to establish a physical map that includes H19 and Igf−2. Igf−2 lies approx90 kilobases of DNA 5' to H19, in the same transcriptional orientation. This physical proximity is conserved in humans, based on pulsed−field gel analysis. We conclude that H19 and Igf−2 constitute an imprinted domain.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalNature Genetics
Volume2
Issue number1
DOIs
StatePublished - Jan 1 1992

All Science Journal Classification (ASJC) codes

  • Genetics

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