Photonic reagents for concentration measurement of flu-orescent proteins with overlapping spectra

Alexei Goun, Denys I. Bondar, Ali O. Er, Zachary Quine, Herschel A. Rabitz

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

By exploiting photonic reagents (i.e., coherent control by shaped laser pulses), we employ Optimal Dynamic Discrimination (ODD) as a novel means for quantitatively characterizing mixtures of fluorescent proteins with a large spectral overlap. To illustrate ODD, we simultaneously measured concentrations of in vitro mixtures of Enhanced Blue Fluorescent Protein (EBFP) and Enhanced Cyan Fluorescent Protein (ECFP). Building on this foundational study, the ultimate goal is to exploit the capabilities of ODD for parallel monitoring of genetic and protein circuits by suppressing the spectral cross-talk among multiple fluorescent reporters.

Original languageEnglish (US)
Article number25827
JournalScientific reports
Volume6
DOIs
StatePublished - May 16 2016

All Science Journal Classification (ASJC) codes

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