Photoenzymatic Reductions Enabled by Direct Excitation of Flavin-Dependent "Ene"-Reductases

Braddock A. Sandoval, Phillip D. Clayman, Daniel G. Oblinsky, Seokjoon Oh, Yuji Nakano, Matthew Bird, Gregory D. Scholes, Todd K. Hyster

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


Non-natural photoenzymatic reactions reported to date have depended on the excitation of electron donor-acceptor complexes formed between substrates and cofactors within protein active sites to facilitate electron transfer. While this mechanism has unlocked new reactivity, it limits the types of substrates that can be involved in this area of catalysis. Here we demonstrate that direct excitation of flavin hydroquinone within "ene"-reductase active sites enables new substrates to participate in photoenzymatic reactions. We found that by using photoexcitation these enzymes gain the ability to reduce acrylamides through a single electron transfer mechanism.

Original languageEnglish (US)
Pages (from-to)1735-1739
Number of pages5
JournalJournal of the American Chemical Society
Issue number4
StatePublished - Feb 3 2021

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry


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