Photocontrol of Smad2, a multiphosphorylated cell-signaling protein, through caging of activating phosphoserines

Michael E. Hahn, Tom W. Muir

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Expressed-protein ligation has been used for the semisynthetic preparation of a Smad2 protein with caged activating phosphoserine residues. Biochemical and cell biological experiments show that the caged protein is activated upon irradiation with UV light (see scheme). This sets the stage for the detailed kinetic analysis of Smad2 function in living cells.

Original languageEnglish (US)
Pages (from-to)5800-5803
Number of pages4
JournalAngewandte Chemie - International Edition
Volume43
Issue number43
DOIs
StatePublished - Nov 5 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Keywords

  • Bioorganic chemistry
  • Phosphorylation
  • Photochemistry
  • Proteins
  • Semisynthesis

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