Photoactivation turns green fluorescent protein red

Michael B. Elowitz, Michael G. Surette, Pierre Etienne Wolf, Jeff Stock, Stanislas Leibler

Research output: Contribution to journalArticlepeer-review

123 Scopus citations

Abstract

In the few years since its gene was first cloned, the Aequorea victoria green fluorescent protein (GFP) has become a powerful tool in cell biology, functioning as a marker for gene expression, protein localization and protein dynamics in living cells [1-3]. GFP variants with improved fluorescence intensity and altered spectral characteristics have been identified, but additional GFP variants are still desirable for multiple labeling experiments, protein interaction studies and improved visibility in some organisms [4]. In particular, long-wavelength (red) fluorescence has remained elusive. Here we describe a red-emitting, green-absorbing fluorescent state of GFP that is generated by photoactivation with blue light. GFP can be switched to its red-emitting state easily with a laser or fluorescence microscope lamp under conditions of low oxygen concentration. This previously unnoticed ability enables regional, non-invasive marking of proteins in vivo. In particular, we report here the use of GFP photoactivation to make the first direct measurements of protein diffusion in the cytoplasm of living bacteria.

Original languageEnglish (US)
Pages (from-to)809-812
Number of pages4
JournalCurrent Biology
Volume7
Issue number10
DOIs
StatePublished - Oct 1 1997

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Photoactivation turns green fluorescent protein red'. Together they form a unique fingerprint.

Cite this