Phase Transitions in Biological Systems with Many Components

William M. Jacobs, Daan Frenkel

Research output: Contribution to journalArticlepeer-review

105 Scopus citations


Biological mixtures such as the cytosol may consist of thousands of distinct components. There is now a substantial body of evidence showing that, under physiological conditions, intracellular mixtures can phase separate into spatially distinct regions with differing compositions. In this article we present numerical evidence indicating that such spontaneous compartmentalization exploits general features of the phase diagram of a multicomponent biomolecular mixture. In particular, we show that demixed domains are likely to segregate when the variance in the intermolecular interaction strengths exceeds a well-defined threshold. Multiple distinct phases are likely to become stable under very similar conditions, which can then be tuned to achieve multiphase coexistence. As a result, only minor adjustments to the composition of the cytosol or the strengths of the intermolecular interactions are needed to regulate the formation of different domains with specific compositions, implying that phase separation is a robust mechanism for creating spatial organization. We further predict that this functionality is only weakly affected by increasing the number of components in the system. Our model therefore suggests that, for purely physico-chemical reasons, biological mixtures are naturally poised to undergo a small number of demixing phase transitions.

Original languageEnglish (US)
Pages (from-to)683-691
Number of pages9
JournalBiophysical Journal
Issue number4
StatePublished - Feb 28 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics


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