TY - JOUR
T1 - Persister formation in Escherichia coli can be inhibited by treatment with nitric oxide
AU - Orman, Mehmet A.
AU - Brynildsen, Mark P.
N1 - Funding Information:
We are grateful to Christina J. DeCoste, John J. Grady, Jessica B. Wiggins, and Jonathan L. Robinson for their technical assistance, and thank the National BioResource Project (NIG, Japan) for their distribution of the Keio collection. This research was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health ( R21AI115075 ), the Department of the Army ( W81XWH-12-2-0138 ), and Princeton University. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health, Department of the Army, or Princeton University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors declare that no competing interests exist.
Publisher Copyright:
© 2016 Elsevier Inc. All rights reserved.
PY - 2016/4
Y1 - 2016/4
N2 - Bacterial persisters are phenotypic variants that survive extraordinary concentrations of antibiotics, and are thought to underlie the propensity of biofilm infections to relapse. Unfortunately many aspects of persister physiology remain ill-defined, which prevents progress toward eradicating the phenotype. Recently, we identified respiration within non-growing Escherichia coli populations as a potential target for the elimination type I persisters, which are those that arise from passage through stationary phase. Here we discovered that nitric oxide (NO) treatment at the onset of stationary phase significantly reduced type I persister formation through its ability to inhibit respiration. NO decreased protein and RNA degradation in stationary phase cells, and produced populations that were more fit for protein synthesis and growth resumption upon introduction into fresh media than untreated controls. Overall, this data shows that NO, which is a therapeutically-relevant compound, has the potential to decrease the incidence of recurrent infections from persisters.
AB - Bacterial persisters are phenotypic variants that survive extraordinary concentrations of antibiotics, and are thought to underlie the propensity of biofilm infections to relapse. Unfortunately many aspects of persister physiology remain ill-defined, which prevents progress toward eradicating the phenotype. Recently, we identified respiration within non-growing Escherichia coli populations as a potential target for the elimination type I persisters, which are those that arise from passage through stationary phase. Here we discovered that nitric oxide (NO) treatment at the onset of stationary phase significantly reduced type I persister formation through its ability to inhibit respiration. NO decreased protein and RNA degradation in stationary phase cells, and produced populations that were more fit for protein synthesis and growth resumption upon introduction into fresh media than untreated controls. Overall, this data shows that NO, which is a therapeutically-relevant compound, has the potential to decrease the incidence of recurrent infections from persisters.
UR - http://www.scopus.com/inward/record.url?scp=84957874170&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957874170&partnerID=8YFLogxK
U2 - 10.1016/j.freeradbiomed.2016.02.003
DO - 10.1016/j.freeradbiomed.2016.02.003
M3 - Article
C2 - 26849946
AN - SCOPUS:84957874170
SN - 0891-5849
VL - 93
SP - 145
EP - 154
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
ER -