Peroxynitrite rapidly permeates phospholipid membranes

Sudhakar S. Marla; Jinbo Lee; John Taylor Groves

doi:10.1073/pnas.94.26.14243

Peroxynitrite rapidly permeates phospholipid membranes

Sudhakar S. Marla, Jinbo Lee, John Taylor Groves

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292 Scopus citations

Abstract

Peroxynitrite (ONOO^-) is a potent oxidant implicated in a number of pathophysiological processes. The activity of ONOO^- is related to its accessibility to biological targets before its spontaneous decomposition (t( 1/4 ) ≃ 1 s at pH 7.4, 37°C). Using model phospholipid vesicular systems and manganese porphyrins as reporter molecules, we demonstrated that ONOO^- freely crosses phospholipid membranes. The calculated permeability coefficient for ONOO^- is ≃ 8.0 x 10^-4 cm · s^-1, which compares well with that of water and is ≃400 times greater than that of superoxide. We suggest that ONOO^- is a significant biological effector molecule not only because of its reactivity but also because of its high diffusibility.

Original language	English (US)
Pages (from-to)	14243-14248
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	94
Issue number	26
DOIs	https://doi.org/10.1073/pnas.94.26.14243
State	Published - Dec 23 1997

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title = "Peroxynitrite rapidly permeates phospholipid membranes",

abstract = "Peroxynitrite (ONOO-) is a potent oxidant implicated in a number of pathophysiological processes. The activity of ONOO- is related to its accessibility to biological targets before its spontaneous decomposition (t( 1/4 ) ≃ 1 s at pH 7.4, 37°C). Using model phospholipid vesicular systems and manganese porphyrins as reporter molecules, we demonstrated that ONOO- freely crosses phospholipid membranes. The calculated permeability coefficient for ONOO- is ≃ 8.0 x 10-4 cm · s-1, which compares well with that of water and is ≃400 times greater than that of superoxide. We suggest that ONOO- is a significant biological effector molecule not only because of its reactivity but also because of its high diffusibility.",

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T1 - Peroxynitrite rapidly permeates phospholipid membranes

AU - Marla, Sudhakar S.

AU - Lee, Jinbo

AU - Groves, John Taylor

PY - 1997/12/23

Y1 - 1997/12/23

N2 - Peroxynitrite (ONOO-) is a potent oxidant implicated in a number of pathophysiological processes. The activity of ONOO- is related to its accessibility to biological targets before its spontaneous decomposition (t( 1/4 ) ≃ 1 s at pH 7.4, 37°C). Using model phospholipid vesicular systems and manganese porphyrins as reporter molecules, we demonstrated that ONOO- freely crosses phospholipid membranes. The calculated permeability coefficient for ONOO- is ≃ 8.0 x 10-4 cm · s-1, which compares well with that of water and is ≃400 times greater than that of superoxide. We suggest that ONOO- is a significant biological effector molecule not only because of its reactivity but also because of its high diffusibility.

AB - Peroxynitrite (ONOO-) is a potent oxidant implicated in a number of pathophysiological processes. The activity of ONOO- is related to its accessibility to biological targets before its spontaneous decomposition (t( 1/4 ) ≃ 1 s at pH 7.4, 37°C). Using model phospholipid vesicular systems and manganese porphyrins as reporter molecules, we demonstrated that ONOO- freely crosses phospholipid membranes. The calculated permeability coefficient for ONOO- is ≃ 8.0 x 10-4 cm · s-1, which compares well with that of water and is ≃400 times greater than that of superoxide. We suggest that ONOO- is a significant biological effector molecule not only because of its reactivity but also because of its high diffusibility.

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Peroxynitrite rapidly permeates phospholipid membranes

Abstract

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