TY - JOUR
T1 - Perforation Does Not Compromise Patterned Two-Dimensional Substrates for Cell Attachment and Aligned Spreading
AU - Bandini, Stephen B.
AU - Spechler, Joshua A.
AU - Donnelly, Patrick E.
AU - Lim, Kelly
AU - Arnold, Craig B.
AU - Schwarzbauer, Jean E.
AU - Schwartz, Jeffrey
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/12/11
Y1 - 2017/12/11
N2 - Polymeric sheets were perforated by laser ablation and were uncompromised by a debris field when first treated with a thin layer of photoresist. Polymer sheets perforated with holes comprising 5, 10, and 20% of the nominal surface area were then patterned in stripes by photolithography, which was followed by synthesis in exposed regions of a cell-attractive zirconium oxide-1,4-butanediphosphonic acid interface. Microscopic and scanning electron microscopy analyses following removal of unexposed photoresist show well-aligned stripes for all levels of these perforations. NIH 3T3 fibroblasts plated on each of these perforated surfaces attached to the interface and spread in alignment with pattern fidelity in every case that is as high as that measured on a nonperforated, patterned substrate.
AB - Polymeric sheets were perforated by laser ablation and were uncompromised by a debris field when first treated with a thin layer of photoresist. Polymer sheets perforated with holes comprising 5, 10, and 20% of the nominal surface area were then patterned in stripes by photolithography, which was followed by synthesis in exposed regions of a cell-attractive zirconium oxide-1,4-butanediphosphonic acid interface. Microscopic and scanning electron microscopy analyses following removal of unexposed photoresist show well-aligned stripes for all levels of these perforations. NIH 3T3 fibroblasts plated on each of these perforated surfaces attached to the interface and spread in alignment with pattern fidelity in every case that is as high as that measured on a nonperforated, patterned substrate.
KW - perforated substrate
KW - polymer laser ablation
KW - two-dimensional patterned cell alignment
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U2 - 10.1021/acsbiomaterials.7b00339
DO - 10.1021/acsbiomaterials.7b00339
M3 - Article
C2 - 33445355
AN - SCOPUS:85038213200
SN - 2373-9878
VL - 3
SP - 3123
EP - 3127
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 12
ER -