Peptide ligation and its application to protein engineering

Graham J. Cotton; Tom W. Muir

doi:10.1016/S1074-5521(99)80109-4

Peptide ligation and its application to protein engineering

Graham J. Cotton, Tom W. Muir

Research output: Contribution to journal › Review article › peer-review

84 Scopus citations

Abstract

The ability to assemble a target protein from a series of peptide fragments, either synthetic or biosynthetic in origin, enables the covalent structure of a protein to be modified in an unprecedented fashion. The present technologies available for performing such peptide ligations are discussed, with an emphasis on how these methodologies have been utilized in protein engineering to investigate biological processes.

Original language	English (US)
Pages (from-to)	R247-R256
Journal	Chemistry and Biology
Volume	6
Issue number	9
DOIs	https://doi.org/10.1016/S1074-5521(99)80109-4
State	Published - Sep 1999
Externally published	Yes

All Science Journal Classification (ASJC) codes

Drug Discovery
Molecular Medicine
Molecular Biology
Biochemistry
Clinical Biochemistry
Pharmacology

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10.1016/S1074-5521(99)80109-4

Cite this

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title = "Peptide ligation and its application to protein engineering",

abstract = "The ability to assemble a target protein from a series of peptide fragments, either synthetic or biosynthetic in origin, enables the covalent structure of a protein to be modified in an unprecedented fashion. The present technologies available for performing such peptide ligations are discussed, with an emphasis on how these methodologies have been utilized in protein engineering to investigate biological processes.",

author = "Cotton, {Graham J.} and Muir, {Tom W.}",

note = "Funding Information: We thank all members of the Muir group for work was supported by the N.I.H. (GM55843, Trusts (T.W.M.) and the Burroughs-Wellcome",

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AU - Cotton, Graham J.

AU - Muir, Tom W.

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Y1 - 1999/9

N2 - The ability to assemble a target protein from a series of peptide fragments, either synthetic or biosynthetic in origin, enables the covalent structure of a protein to be modified in an unprecedented fashion. The present technologies available for performing such peptide ligations are discussed, with an emphasis on how these methodologies have been utilized in protein engineering to investigate biological processes.

AB - The ability to assemble a target protein from a series of peptide fragments, either synthetic or biosynthetic in origin, enables the covalent structure of a protein to be modified in an unprecedented fashion. The present technologies available for performing such peptide ligations are discussed, with an emphasis on how these methodologies have been utilized in protein engineering to investigate biological processes.

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DO - 10.1016/S1074-5521(99)80109-4

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JO - Chemistry and Biology

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Peptide ligation and its application to protein engineering

Abstract

All Science Journal Classification (ASJC) codes

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