Peptide ligation and its application to protein engineering

Graham J. Cotton, Tom W. Muir

Research output: Contribution to journalReview articlepeer-review

84 Scopus citations


The ability to assemble a target protein from a series of peptide fragments, either synthetic or biosynthetic in origin, enables the covalent structure of a protein to be modified in an unprecedented fashion. The present technologies available for performing such peptide ligations are discussed, with an emphasis on how these methodologies have been utilized in protein engineering to investigate biological processes.

Original languageEnglish (US)
Pages (from-to)R247-R256
JournalChemistry and Biology
Issue number9
StatePublished - Sep 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Molecular Medicine
  • Molecular Biology
  • Biochemistry
  • Clinical Biochemistry
  • Pharmacology


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