Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain

Julio A. Camarero, David Fushman, David Cowburn, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Here we describe the first example of a peptide chemical ligation reaction performed inside a living cell. A cell-based native chemical ligation approach was developed and used to generate a circular version of the N-terminal Src homology 3 (SH3) domain from the murine c-Crk adapter protein inside Escherichia coli cells. The in vivo cyclization reaction was extremely efficient and the resulting circular protein domain was fully biologically active and able to adopt the native SH3 folded structure. This work represents an important step towards the in vivo generation of small backbone cyclic peptides for use in basic biological research.

Original languageEnglish (US)
Pages (from-to)2479-2484
Number of pages6
JournalBioorganic and Medicinal Chemistry
Volume9
Issue number9
DOIs
StatePublished - Sep 22 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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