TY - JOUR
T1 - Patterning potential of the terminal system in the Drosophila embryo
AU - Lee, Keonyong
AU - O’Neill, Kate Molloy
AU - Ku, Jayoung
AU - Shvartsman, Stanislav Yefimovic
AU - Kim, Yoosik
N1 - Publisher Copyright:
© 2023, Korean Institute of Chemical Engineering (KIChE).
PY - 2023/2
Y1 - 2023/2
N2 - Segmentation of the Drosophila embryo is initiated by localized maternal signals. In this context, anteriorly localized Bicoid activates the gap genes in the anterior half of the embryo while posteriorly localized Nanos represses the translation of maternal hunchback mRNA to pattern the posterior half. The non-segmented termini are patterned by the localized activation of mitogen-activated protein kinase. Yet, the spatial extent of the terminal patterning system in regulating gap genes beyond poles remains unknown. We investigated the patterning potential of the terminal system using mutagenized embryos that lack both the anterior and the posterior maternal signaling systems. Using a combination of quantitative imaging and mathematical modeling, we analyzed the spatial patterns of gap genes in the early Drosophila embryo. We found that this mutant embryo develops symmetric cuticle patterns along the anteroposterior axis with two segments on each side. Notably, the terminal system can affect the expression of Krüppel in the torso region. Our mathematical model recapitulates the experimental data and reveals the potential bistability in the terminal patterning system. Collectively, our study suggests that the terminal system can act as a long-range inductive signal and establish multiple gene expression boundaries along the anteroposterior axis of the developing embryo.
AB - Segmentation of the Drosophila embryo is initiated by localized maternal signals. In this context, anteriorly localized Bicoid activates the gap genes in the anterior half of the embryo while posteriorly localized Nanos represses the translation of maternal hunchback mRNA to pattern the posterior half. The non-segmented termini are patterned by the localized activation of mitogen-activated protein kinase. Yet, the spatial extent of the terminal patterning system in regulating gap genes beyond poles remains unknown. We investigated the patterning potential of the terminal system using mutagenized embryos that lack both the anterior and the posterior maternal signaling systems. Using a combination of quantitative imaging and mathematical modeling, we analyzed the spatial patterns of gap genes in the early Drosophila embryo. We found that this mutant embryo develops symmetric cuticle patterns along the anteroposterior axis with two segments on each side. Notably, the terminal system can affect the expression of Krüppel in the torso region. Our mathematical model recapitulates the experimental data and reveals the potential bistability in the terminal patterning system. Collectively, our study suggests that the terminal system can act as a long-range inductive signal and establish multiple gene expression boundaries along the anteroposterior axis of the developing embryo.
KW - Bistability
KW - Embryogenesis
KW - Gap Genes
KW - MAPK
KW - Signal Transduction
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U2 - 10.1007/s11814-022-1298-6
DO - 10.1007/s11814-022-1298-6
M3 - Article
AN - SCOPUS:85147363718
SN - 0256-1115
VL - 40
SP - 436
EP - 444
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
IS - 2
ER -