Overexpression and characterization of an iron storage and DNA-binding Dps protein from Trichodesmium erythraeum

M. Castruita, M. Saito, P. C. Schottel, L. A. Elmegreen, Satish Chandra Babu Myneni, E. I. Stiefel, Francois M. M. Morel

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Although the role of iron in marine productivity has received a great deal of attention, no iron storage protein has been isolated from a marine microorganism previously. We describe an Fe-binding protein belonging to the Dps family (DNA binding protein from starved cells) in the N2-fixing marine cyanobacterium Trichodesmium erythraeum. A dps gene encoding a protein with significant levels of identity to members of the Dps family was identified in the genome of T. erythraeum. This gene codes for a putative Dps T. erythraeurm protein (Dpstery) with 69% primary amino acid sequence similarity to Synechococcus DpsA. We expressed and purified Dpstery, and we found that Dpstery, like other Dps proteins, is able to bind Fe and DNA and protect DNA from degradation by DNase. We also found that Dpstery binds phosphate, like other ferritin family proteins. Fe K near-edge X-ray absorption of Dpstery indicated that it has an iron core that resembles that of horse spleen ferritin.

Original languageEnglish (US)
Pages (from-to)2918-2924
Number of pages7
JournalApplied and Environmental Microbiology
Volume72
Issue number4
DOIs
StatePublished - Apr 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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