Overcoming component limitations in synthetic biology through transposon-mediated protein engineering

Joshua T. Atkinson, Bingyan Wu, Laura Segatori, Jonathan J. Silberg

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations


Protein fission and fusion can be used to create biomolecules with new structures and functions, including circularly permuted proteins that require post-translational modifications for activity, split protein AND gates that require multiple inputs for activity, and fused domains that function as chemical-dependent protein switches. Herein we describe how transposon mutagenesis can be used for protein design to create libraries of permuted, split, or domain-inserted proteins. When coupled with a functional screen or selection, these approaches can rapidly diversify the topologies and functions of natural proteins and create useful protein components for synthetic biology.

Original languageEnglish (US)
Title of host publicationChemical and Synthetic Biology Approaches To Understand Cellular Functions - Part A
EditorsArun K. Shukla
PublisherAcademic Press Inc.
Number of pages22
ISBN (Print)9780128181171
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


  • Combinatorial library
  • Deep sequencing
  • Mutagenesis
  • Protein engineering
  • Synthetic biology
  • Transposon


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