@inbook{b0d8f33a1977493cb8a9079f80691e42,
title = "Overcoming component limitations in synthetic biology through transposon-mediated protein engineering",
abstract = "Protein fission and fusion can be used to create biomolecules with new structures and functions, including circularly permuted proteins that require post-translational modifications for activity, split protein AND gates that require multiple inputs for activity, and fused domains that function as chemical-dependent protein switches. Herein we describe how transposon mutagenesis can be used for protein design to create libraries of permuted, split, or domain-inserted proteins. When coupled with a functional screen or selection, these approaches can rapidly diversify the topologies and functions of natural proteins and create useful protein components for synthetic biology.",
keywords = "Combinatorial library, Deep sequencing, Mutagenesis, Protein engineering, Synthetic biology, Transposon",
author = "Atkinson, {Joshua T.} and Bingyan Wu and Laura Segatori and Silberg, {Jonathan J.}",
note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
doi = "10.1016/bs.mie.2019.02.014",
language = "English (US)",
isbn = "9780128181171",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "191--212",
editor = "Shukla, {Arun K.}",
booktitle = "Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part A",
address = "United States",
}