Order-dependent coincidence detection in cerebellar Purkinje neurons at the inositol trisphosphate receptor

Dmitry V. Sarkisov, Samuel S.H. Wang

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Associative long-term depression (LTD) at cerebellar parallel fiber-Purkinje cell synapses is sensitive to the temporal order in which the parallel fiber is coactivated with the climbing fiber input, but how order sensitivity is achieved is unknown. Here we show that the cerebellar inositol-1,4,5-trisphosphate (IP3) receptor, whose activation is required for LTD induction, is sensitive in situ to the order of presentation of its coagonists, IP3 and cytoplasmic calcium. By focally photolyzing a novel caged IP3 compound in dendritic spines, we find that pairing IP3 with climbing fiber-mediated calcium entry leads to a large calcium release transient if the climbing fiber is activated up to 100 ms before or up to 500 ms after IP3 uncaging. This asymmetric timing window for coactivation follows the kinetics of calcium removal and IP3 unbinding from the receptor and is not limited by IP3 metabolism. IP3 receptor binding thus acts as an eligibility trace that can drive temporal order-dependent calcium release and LTD induction in Purkinje cells and event order-dependent sensory plasticity in the whole animal.

Original languageEnglish (US)
Pages (from-to)133-142
Number of pages10
JournalJournal of Neuroscience
Volume28
Issue number1
DOIs
StatePublished - Jan 2 2008

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • Calcium channels
  • Coincidence detection
  • Inositol trisphosphate receptor
  • Ip
  • LTD (long-term depression)
  • Plasticity
  • Purkinje neurons

Fingerprint Dive into the research topics of 'Order-dependent coincidence detection in cerebellar Purkinje neurons at the inositol trisphosphate receptor'. Together they form a unique fingerprint.

Cite this