Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation

Anand P. Singh, Ping Wu, Sergey Ryabichko, João Raimundo, Michael Swan, Eric Wieschaus, Thomas Gregor, Jared E. Toettcher

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Developmental patterning networks are regulated by multiple inputs and feedback connections that rapidly reshape gene expression, limiting the information that can be gained solely from slow genetic perturbations. Here we show that fast optogenetic stimuli, real-time transcriptional reporters, and a simplified genetic background can be combined to reveal the kinetics of gene expression downstream of a developmental transcription factor in vivo. We engineer light-controlled versions of the Bicoid transcription factor and study their effects on downstream gap genes in embryos. Our results recapitulate known relationships, including rapid Bicoid-dependent transcription of giant and hunchback and delayed repression of Krüppel. In addition, we find that the posterior pattern of knirps exhibits a quick but inverted response to Bicoid perturbation, suggesting a noncanonical role for Bicoid in directly suppressing knirps transcription. Acute modulation of transcription factor concentration while recording output gene activity represents a powerful approach for studying developmental gene networks in vivo.

Original languageEnglish (US)
Article number110543
JournalCell Reports
Volume38
Issue number12
DOIs
StatePublished - Mar 22 2022

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • Bicoid
  • CP: Cell Biology
  • Drosophila development
  • gap gene network
  • kinetics
  • live imaging
  • optogenetics
  • transcription

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