Optimizing the detection of nascent transcripts by RNA fluorescence in situ hybridization

Catherine D. Van Raamsdonk, Shirley M. Tilghman

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

An unusual feature of the mammalian genome is the number of genes exhibiting monoallelic expression. Recently random monoallelic expression of autosomal genes has been reported for olfactory and Ly-49 NK receptor genes, as well as for Il-2, Il-4 and Pax5. RNA fluorescence in situ hybridization (FISH) has been exploited to monitor allelic expression by visualizing the number of sites of transcription in individual nuclei. However, the sensitivity of this technique is difficult to determine for a given gene. We show that by combining DNA and RNA FISH it is possible to control for the hybridization efficiency and the accessibility and visibility of fluorescent probes within the nucleus.

Original languageEnglish (US)
Article numbere42
JournalNucleic acids research
Volume29
Issue number8
DOIs
StatePublished - 2020

All Science Journal Classification (ASJC) codes

  • Genetics

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