On the GFP-based analysis of dynamic concentration profiles

Alexander M. Berezhkovskii; Stanislav Y. Shvartsman

doi:10.1016/j.bpj.2013.12.007

On the GFP-based analysis of dynamic concentration profiles

Alexander M. Berezhkovskii, Stanislav Y. Shvartsman

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6 Scopus citations

Abstract

Studies with GFP-tagged proteins can be used to investigate the dynamics of concentration profiles of regulatory proteins in cells and tissues. Analysis of these experiments must account for the finite rate with which the GFP-tagged proteins mature to the fluorescent state. Toward this end, we present an analytical framework that provides an explicit connection between the apparent kinetics of concentration gradients and the rates of GFP maturation.

Original language	English (US)
Pages (from-to)	L13-L15
Journal	Biophysical Journal
Volume	106
Issue number	3
DOIs	https://doi.org/10.1016/j.bpj.2013.12.007
State	Published - Feb 4 2014

All Science Journal Classification (ASJC) codes

Biophysics

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10.1016/j.bpj.2013.12.007

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AB - Studies with GFP-tagged proteins can be used to investigate the dynamics of concentration profiles of regulatory proteins in cells and tissues. Analysis of these experiments must account for the finite rate with which the GFP-tagged proteins mature to the fluorescent state. Toward this end, we present an analytical framework that provides an explicit connection between the apparent kinetics of concentration gradients and the rates of GFP maturation.

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On the GFP-based analysis of dynamic concentration profiles

Abstract

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