On the GFP-based analysis of dynamic concentration profiles

Alexander M. Berezhkovskii, Stanislav Y. Shvartsman

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Studies with GFP-tagged proteins can be used to investigate the dynamics of concentration profiles of regulatory proteins in cells and tissues. Analysis of these experiments must account for the finite rate with which the GFP-tagged proteins mature to the fluorescent state. Toward this end, we present an analytical framework that provides an explicit connection between the apparent kinetics of concentration gradients and the rates of GFP maturation.

Original languageEnglish (US)
Pages (from-to)L13-L15
JournalBiophysical Journal
Volume106
Issue number3
DOIs
StatePublished - Feb 4 2014

All Science Journal Classification (ASJC) codes

  • Biophysics

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