TY - JOUR
T1 - Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling
AU - Miyasaka, Nobuhiko
AU - Arganda-Carreras, Ignacio
AU - Wakisaka, Noriko
AU - Masuda, Miwa
AU - Sümbül, Uygar
AU - Seung, Hyunjune Sebastian
AU - Yoshihara, Yoshihiro
N1 - Funding Information:
We thank Rainer Friedrich and Charles Yokoyama for critical reading of the manuscript, Koichi Kawakami for reagents and transgenic fish, Shankar Srinivas and Stephen J. Smith for reagents, Toshiyuki Shiraki, Hisaya Kakinuma and Hitoshi Okamoto for assistance in generation and maintenance of transgenic fish, Tetsuya Koide for help in identification of zebrafish tbx21 gene and members of the Yoshihara laboratory for fish care and discussion. This work was supported in part by Grants-in-Aid for Scientific Research (22500303 and 25430025 to N.M.; 20300117 to Y.Y.) and for Scientific Research on Innovative Areas ‘Fluorescence Live Imaging’ (23113520 and 25113724 to N.M.), ‘Systems Molecular Ethology’ (23115723 to Y.Y.) and ‘Memory Dynamism’ (25115005 to Y.Y.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and a Grant from the Human Frontier Science Program (HFSP RGP0015/2010 to H.S.S. and Y.Y.).
PY - 2014/4/9
Y1 - 2014/4/9
N2 - Chemotopic odour representations in the olfactory bulb are transferred to multiple forebrain areas and translated into appropriate output responses. However, a comprehensive projection map of bulbar output neurons at single-axon resolution is lacking in vertebrates. Here we unravel a projectome of the zebrafish olfactory bulb through genetic single-neuron tracing and image registration. We show that five major target regions receive distinct modes of projections from olfactory bulb glomeruli. The central portion of posterior telencephalon receives non-selective, interspersed inputs from all glomeruli, whereas the ventral telencephalon is diffusely innervated by axons from particular glomerular clusters. The right habenula and posterior tuberculum (diencephalic nuclei) receive convergent inputs from restricted and all glomerular clusters, respectively. The bulbar recurrent projections are coarsely topographic. Thus, the primary chemotopic organization is transformed into distinct sensory representations in higher olfactory centres. These findings provide a framework to understand general principles as well as species-specific features in decoding of odour information.
AB - Chemotopic odour representations in the olfactory bulb are transferred to multiple forebrain areas and translated into appropriate output responses. However, a comprehensive projection map of bulbar output neurons at single-axon resolution is lacking in vertebrates. Here we unravel a projectome of the zebrafish olfactory bulb through genetic single-neuron tracing and image registration. We show that five major target regions receive distinct modes of projections from olfactory bulb glomeruli. The central portion of posterior telencephalon receives non-selective, interspersed inputs from all glomeruli, whereas the ventral telencephalon is diffusely innervated by axons from particular glomerular clusters. The right habenula and posterior tuberculum (diencephalic nuclei) receive convergent inputs from restricted and all glomerular clusters, respectively. The bulbar recurrent projections are coarsely topographic. Thus, the primary chemotopic organization is transformed into distinct sensory representations in higher olfactory centres. These findings provide a framework to understand general principles as well as species-specific features in decoding of odour information.
UR - http://www.scopus.com/inward/record.url?scp=84898458262&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898458262&partnerID=8YFLogxK
U2 - 10.1038/ncomms4639
DO - 10.1038/ncomms4639
M3 - Article
C2 - 24714622
AN - SCOPUS:84898458262
SN - 2041-1723
VL - 5
JO - Nature communications
JF - Nature communications
M1 - 3639
ER -